Cytotoxic Effects of <i>Artemisia Dracunculus</i> L. and <i>Heracleum Persicum</i> Desf. Extracts on <i>Leishmania major</i> and <i>Leishmania infantum</i> Promastigotes Using MTT Assay

Background: Leishmaniasis is a parasitic disease that occurs in subtropical and tropical regions with approximately 350 million people worldwide 2 new cases annually. The annual increase cutaneous leishmaniasis (CL) observed, especially endemic areas such as Iran. Since there no effective vaccine, the detection of natural anti-leishmanial products essential. Objective: purpose this study was to evaluate vitro activity two herbal medicine including Artemisia dracunculus L. Heracleum persicum Desf. (Golpar). Materials Methods: extracts selected plants were obtained by maceration, assayed on Leishmania major infantum promastigotes using colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay comparison glucantime reference. Results: Based results, 50% inhibitory concentration (IC50) values solutions determined at 24, 48, 72 hours incubation. Further, leaf extract A. IC50 1.85 3.5 µg/mL fruit H. 31.32 11.7 evaluated against promastigotes, respectively. Conclusion: These results revealed anti-leishmania properties above-mentioned need effects these genus animal models vivo future.